Survivals of human ADSCs transplanted into zebrafish embryos.

<p>The GFP-expressing human ADSCs were transplanted into zebrafish embryos at 3.3–4.3 hpf after their chorions were removed, and followed by observation under fluorescence microscope at different time points. Each embryo was administered with about 10 cells, but the control did not receive cell transplantation and only their chorions were removed. (A1-D1) The representative control images of a same embryo standing for typical development stages at 12, 24, 48, and 96 hpf (bright). (A2-D2) The representative images of a same embryo captured under fluorescence microscope at indicated time points (bright + fluorescence). Original magnification: 40 ×. The white arrows indicate the location of GFP-expressing ADSCs (green) in the embryos and zebrafish. The insets in (C2) and (D2) show the enlarged images of transplanted GFP-expressing cells in the zebrafish. hpf: hour post-fertilization.