The effects of 10 days of intraperitoneal FL on DC and Treg populations.

<p>(A) Experimental design, where FL or PBS was administered intraperitoneally to mice for daily for 10 days. (B) Total spleen and pooled LN cell number. (C) Representative FACS plots showing proportions of pDCs (CD11c+PDCA-1+ cells) in the spleen, gating on all CD11c+ leukocytes. (D) Proportions of CD11c+ cells in the spleen and LN. (E) Proportions of pDCs in the spleen and LN. (F) Representative FACS plots showing Tregs in the spleen, gating on lymphocytes, staining for CD4+ and foxp3+ cells. (G) Proportion of Tregs in the spleen and LN. Black bars represent PBS treated mice. White bars represent FL treated mice. n = 4 per group. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001.