Study of the in vivo effect of oseltamivir treatment in CMT-U27 xenografts in female nude mice.

<p>CMT-U27 cells were xenografted into the mammary fat pad of 8 mice and divided in two groups according to treatment. Mice were treated daily (IP) with either 100mg/kg oseltamivir or PBS. Tumors were exised when reaching 1000–1500 mm³. (A) Oseltamivir-treated mice presented a more prominent inflammatory infiltrate when compared with non-treated animals. Representative images of Ki-67 (B) and caspase-3 (C) expression (brown colour). Histochemical staining with SNA (D) and MALI (E) lectins (brown colour) was used to study terminal α2,6 and Siaα2,3Galβ1-4GlcNAc sialic acid structures in CMT-U27 xenografts. Increased expression in α2,6 sialic acid terminal structures (<i>p</i> = 0.07) was observed in oseltamivir-treated when compared to non-treated mice. Overall siaα2-3Galβ1-4GlcNAc sialic acids structures (MALI) were less altered. (F) SLe(x) expression was also slightly increased in oseltamivir-treated when compared to non-treated mice. (G) Thorough analysis of lung tissues from both groups of mice showed a higher number of lung metastases in oseltamivir-treated when compared to non-treated animals (arrows).