Analysis of sialylated glycan structures in canine mammary tumor cell lines treated with oseltamivir.

<p>(A) Expression of sialylated structures was evaluated by blot analysis using different lectins and monoclonal antibodies. Detection of α2,6 sialylated structures by SNA lectin blot showed increased expression of these structures in oseltamivir phosphate-treated CMA07 cells (0.305 μM, 3.05 μM and 30.5 μM), when compared control cells, mainly in the region of the 120 KDa molecular weight region. Analysis of SLe(x) and STn structures also demonstrated that they were increased in protein extracts from oseltamivir phosphate-treated cells. (B) 2D analysis of glycoproteins labeled with SNA better demonstrated an increased expression of α2,6 sialylated structures in 3.05 μM oseltamivir phosphate-treated cells when compared with PBS-treated cells. Finally, the broader expression of terminal α2,6 sialic acids structures was evaluated by SNA lectin fluorescent labelling upon oseltamivir phosphate treatment. Oseltamivir phosphate-treated CMT-U27 and CMA07 cells (3.05 μM) presented increased α2,6 sialic acids structures when compared with control cells (x20 magnification).