Expression of TLR4, NF-κB p65 and cytokines in human normal pregnancy and preeclampsia.

<p>(A-C), Immunohistochemistry for TLR4 (A) and NF-κB p65 (B) in paraffin-embedded placental tissue sections (4 μm) from human normal pregnancy and preeclampsia. Corresponding concentrations of anti-IgG served as non-specific controls (C). Magnification 400 ×. Bar 100 μm. (D-E), mRNA levels of IL-6 (D) and MCP-1 (E) in the placenta from normal pregnancy and preeclampsia. IL-6 and MCP-1 mRNA levels were measured by qRT-PCR and normalized to that of GAPDH. (F-G) Serum levels of IL-6 (F) and MCP-1(G) in human normal pregnancy and preeclampsia were quantitatively measured by ELISA. Data are expressed by mean ± SEM, N = 10. *Means <i>P</i> < 0.05.