Effect of netrin-4 on HUVECs migration and invasion.

<p>HUVEC were treated during 24 h in medium without serum, in the presence of EBM2 alone or EBM2 with different concentrations of netrin-4. (A)Pictures were taken at 0 h and 24 h after insert removing. (B) Results are expressed as percentage of wound closure (percentage closure) (mean ± SD). (C) Netrin-4 affects HUVEC migration in a transwell assay. HUVEC cells were allowed to migrate for 24 h at 37°C in a 5% CO₂ humidified incubator. After 24 h, the cells that migrated across the membrane were stained with DAPI and the fluorescence intensity was measured. (D)The % invasion is compared to the fluorescence intensity of migrated cells in the absence of netrin-4 (** <i>p</i> < 0.01). Each experiment was performed three times and a representative picture of each condition is shown. Data are expressed as mean ± SD.