Mitochondrial superoxide levels in the hippocampus of 6-month-old mice.

<p>Mitochondrial superoxide production in hippocampal slices (400 μm) was stained employing MitoSOX Red solution (shown in red color). Nuclei were counterstained with DAPI (shown in blue). (A) Bar graphs show comparison of mitochondrial superoxide levels between control strains (BL6 and mtAKR) and conplastic mutant strains (mtALR and mt129S1). Each bar represents mean fluorescence intensity (MFI) of MitoSOX Red as percentage to MFI of DAPI. All data consist of n = 5. (*p < 0.05). (B) Fluorescence microscopy of pyramidal cells from CA1 region of BL6 (B₁), mtAKR (B₂), mtALR (B₃) and mt129S1 (B₄). Pictures were taken with 120x magnification.