Exogenous VEGF partially rescues normal cell phenotype in α-CaMKII silenced 143B OS cells.

<p>143B cells were transduced with lentiviruses expressing either scrambled (shCtrl) or α-CaMKII- targeting shRNAs (shCaMKIIα) <b>A.</b> MTT assay (left panel) was performed at days 1, 2, 3, 4 and 5 to determine the number of viable cells. 5x10³ 143B cells were seeded in a 96-well plate. At 24 hours after seeding, cells were treated with 100nM VEGF. Values were obtained from three separate experiments, each repeated in triplicate and represent the mean ± S.D. *p<0.01. Trypan blue exclusion assay (right panel) was performed at day 5 to determine the live cell number. At 24 hours after seeding, cells were treated with 100nM VEGF every 24 hours. Values were obtained from three separate experiments, each repeated in triplicate and represent the mean ± S.D. *p<0.01. <b>B.</b> Scratch/wound healing assay was performed on cells cultured for 12 hours. The width between the scratched areas at hour 0 was set to 100%. Representative photomicrographs were taken at 50x magnification from 3 independent experiments, each repeated in triplicate. Values represent the mean ± S.D. *p<0.01. <b>C</b>. Trans-well invasion assay allowing cells to invade for 24 hours. Representative photomicrographs were taken at 100x magnifications from 3 independent experiments, each repeated in duplicate. Values represent the mean ± S.D. *p<0.01.