<p>(A and B) Dzip1 interacts with GSK3β. Endogenous GSK3β was immunoprecipitated by Dzip1 but not IgG (A), and endogenous Dzip1 was immunoprecipitated with GFP-GSK3β in HEK 293T cells (B). (C and D) Dzip1 is co-localized with GSK3β at the basal body. G0-phase NIH 3T3 cells expressing GFP-GSK3β were immunostained for Dzip1 and AcTub (C), or cells expressing BFP-Centrin2 were immunostained with GSK3β and Dzip1 (D). Scale bar: 5 μm. (E) GSK3β binds Dzip1 in a kinase-substrate interaction manner. Wild-type (WT) GFP-GSK3β and the mutants S9A, K85R, and R96A were each co-expressed with Myc-Dzip1 in G0-phase HEK 293T cells, and treated with the CK1 inhibitor D4476 or the CK2 inhibitor CX4945. Note that treatment with CX4945 but not D4476 led to a ...
<p>(A and B) Knockdown of Dzip1 impairs ciliary localization of Rab8. RNAi control (Con) and Dzip1 s...
The primary cilium, which disassembles before mitotic entry and reassembles after mitosis, organizes...
GSK3beta interaction protein (GSKIP) is a negative regulator of GSK3B (GSK3 beta) which is a highly ...
<p>(A) The binding of Dzip1 with GDI2 is prevented by inhibition of GSK3. G0-phase NIH 3T3 cells wer...
<p>Drp1 is phosphorylated by GSK3beta. (A) Purified GSK3beta was used in an <i>in vitro</i> kinase a...
<p>(A) Schematic representation of the USF2 deletion mutants used to identify the domains that are p...
<p>(A) Examination of the Dzip1 antibody. The antibody Mid2 targeting aa 594–610 of full-length huma...
GSK-3, a well-known serine/threonine kinase is one of the key players controlling numerous cellular ...
<p>(<b>A</b>) GSK3β, NM1 and actin are co-precipitated from nuclear protein extracts prepared from g...
A-kinase anchoring proteins (AKAPs) represent a family of structurally diverse proteins, all of whic...
Identified originally as a regulator of glycogen metabolism, glycogen synthase kinase-3 (GSK3) is no...
Dynein is a microtubule minus-end directed molecular motor, participating in a broad range of cellul...
<p>Western blot of total GSK-3β and GSK-3β phosphorylation at ser9 (a: blot, b: quantification of ph...
<p>(A) Schematic model of the acquisition of synchronized mitosis–G0 phase NIH 3T3 cells, treated wi...
The oncoprotein BCL-3 is a nuclear transcription factor that activates NF-kappaB target genes throug...
<p>(A and B) Knockdown of Dzip1 impairs ciliary localization of Rab8. RNAi control (Con) and Dzip1 s...
The primary cilium, which disassembles before mitotic entry and reassembles after mitosis, organizes...
GSK3beta interaction protein (GSKIP) is a negative regulator of GSK3B (GSK3 beta) which is a highly ...
<p>(A) The binding of Dzip1 with GDI2 is prevented by inhibition of GSK3. G0-phase NIH 3T3 cells wer...
<p>Drp1 is phosphorylated by GSK3beta. (A) Purified GSK3beta was used in an <i>in vitro</i> kinase a...
<p>(A) Schematic representation of the USF2 deletion mutants used to identify the domains that are p...
<p>(A) Examination of the Dzip1 antibody. The antibody Mid2 targeting aa 594–610 of full-length huma...
GSK-3, a well-known serine/threonine kinase is one of the key players controlling numerous cellular ...
<p>(<b>A</b>) GSK3β, NM1 and actin are co-precipitated from nuclear protein extracts prepared from g...
A-kinase anchoring proteins (AKAPs) represent a family of structurally diverse proteins, all of whic...
Identified originally as a regulator of glycogen metabolism, glycogen synthase kinase-3 (GSK3) is no...
Dynein is a microtubule minus-end directed molecular motor, participating in a broad range of cellul...
<p>Western blot of total GSK-3β and GSK-3β phosphorylation at ser9 (a: blot, b: quantification of ph...
<p>(A) Schematic model of the acquisition of synchronized mitosis–G0 phase NIH 3T3 cells, treated wi...
The oncoprotein BCL-3 is a nuclear transcription factor that activates NF-kappaB target genes throug...
<p>(A and B) Knockdown of Dzip1 impairs ciliary localization of Rab8. RNAi control (Con) and Dzip1 s...
The primary cilium, which disassembles before mitotic entry and reassembles after mitosis, organizes...
GSK3beta interaction protein (GSKIP) is a negative regulator of GSK3B (GSK3 beta) which is a highly ...