Phosphorylation of the <i>Mtb</i> MDH by <i>Mtb</i> STPKs during various bacterial growth conditions.

<p>The immunoprecipitated MDH was separated on SDS-PAGE and transferred onto nitrocellulose membranes for Western blot analyses using antibodies recognizing the MDH (anti-MDH) or phosphothreonine residue (anti-pThr). A: Bacteria cultured until early, middle and late exponential phases, (corresponding to OD_600nm 0.3, 0.6 or 0.9, respectively) were further cultured for 3 hours in phosphate-repleted medium or bacteria cultured until late exponential phase (corresponding to OD_600nm 0.9) were further cultured in phosphate limited medium. B: Bacteria cultured until OD_600nm 0.6 in Dubos normal medium (DM) or under deprivation of oxygen (DM-O₂).