Validation of COL1A1 and NCAM1 expression in 20 pairs of gastric cancer and normal tissues.

<p>A and B, Detection of COL1A1 and NCAM1 mRNA expression in gastric cancer vs. normal tissues using PCR and qRT-PCR. Levels of COL1A1, NCAM1 mRNA were 3.10 ± 1.08 folds up-regulation and 0.37 ± 0.02 folds down-regulation in tumor tissues, respectively compared to those of the normal ones. *p<0.01. C and D, Western blot analysis of COL1A1 protein. Tumor tissues expressed higher level of COL1A1 protein compared to the normal ones (p<0.01). E and F, Western blot analysis of NCAM1 protein. Tumor tissues expressed lower level of NCAM1 protein compared to the normal ones (p<0.01). N, normal tissues; C, cancer tissues.