GST.

<p><b>(a-d) ezrinABD interaction</b>: (a) binding of bright ezrinABD to SLB, (b) Predicted structure of GST-tagged bright ezrinABD, (c) images from TIRF microscopy, (d) graphical presentation of cy3 Actin binding to Ezrin in different time frame; <b>(e, f) Saturation of ezrinABD and actin binding</b>: (e) TIRF microscopy image of f-actin binding to SLB via HKE (dark ezrinABD). (f) Plot of actin binding (area fraction covered by actin) at different concentrations of HKE; red line is a fit of a logistic function, to visualize the sharp onset of actin binding to the SLB at C_HKE > 0.7 nM, data points represent mean and standard deviation of individual experiments.