Replication of 3′PPT and cPPT mutant viruses.
- Jason W. Rausch (66803)
- Meijuan Tian (402221)
- Yuejin Li (410414)
- Lora Angelova (720443)
- Bernard S. Bagaya (720444)
- Kendall C. Krebs (720445)
- Feng Qian (73252)
- Chuanwu Zhu (720446)
- Eric J. Arts (220561)
- Stuart F. J. Le Grice (6613)
- Yong Gao (29929)
Publication date
April 2015
DOIAbstract
<p>(A) Introduction of single and double mutations into the 3′PPT or cPPT region in an NL4-3 backbone through yeast based/HIV-1 cloning technology [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0122953#pone.0122953.ref033" target="_blank">33</a>]; (B) Replication of various PPT mutants, as well as Δnef mutant (with premature stop codon in nef but outside of PPT region) in U87.CD4.CXCR4 cells. The input viruses were normalized according to RT activity.</p
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