Effect of annomuricin E on Bax and Bcl-2 mRNA expression was assessed using Q-PCR analysis.

<p>The housekeeping gene β-actin was used for the normalization of the mRNA expression. The result depicted a time-dependent upregulation of Bax and down-regulation of Bcl-2 after treatment with annomuricin E at the IC₅₀ concentration. The data represent the means ± SEM of three independent experiments. *<i>P</i><0.05 compared with the control.