Effect of annomuricin E on cell cycle distribution in HT-29 cells.

<p>Cells were treated with (A) 0.1% vehicle DMSO (control) for 48 h and annomuricin E at the IC₅₀ concentration for (B) 12, (C) 24 and (D) 48 h. After staining the cells with PI, the DNA contents were monitored using flow cytometry. (E) The representative bar chart shows the significant induction of G₁ cell cycle arrest by annomuricin E after 12 h of treatment. The data represent the means ± SEM of three independent experiments. *<i>P</i><0.05 compared with the control.