Effect of 15d-PGJ₂ on osteolytic bone metastasis in nude mice that received intracardiac injections of MDA-MB-231 cells.

<p>MDA-MB-231/Luc⁺ cells were inoculated into the left ventricles of female nude mice. 15d-PGJ₂ or zoledronic acid (ZA) was subcutaneously injected 3 times per week for 6 weeks at the indicated doses (<i>n</i> = 10). (A) Metastatic progression was detected by measuring bioluminescence in the same mice at 3 and 5 weeks after the injection of cancer cells. The formed metastases were quantified by measuring total photon flux per second. (B) Radiographic images of mandibles, distal femora, and proximal tibiae were scanned using μCT 6 weeks after the injection of cancer cells. Arrowheads indicate osteolytic lesions. (C) The mandibles of mice were analyzed using 3D-images. (D) Bone morphometric parameters, including BV/TV, Tb.N, Tb.Th, Tb.Sp, and SMI, were measured using μCT analysis of the proximal tibiae from mice. (E) Serum PTHrP levels were assayed using a commercially available ELISA kit. Data are expressed as the means ± SEM. ^#<i>P</i><0.05, ^##<i>P</i><0.01 vs. control group.*<i>P</i><0.05, **<i>P</i><0.01 vs. vehicle-treated group inoculated with cancer cells.