Overexpression of MiR-124 Suppresses LNCaP Cells Proliferation, Migration and Xenograft Tumors Growth.

<p>(A) LNCaP-control cells and LNCaP-miR-124 cells were plated in 96 wells and examined at 48, 72, 96 hours using CCK8 assay. Cell proliferation was assayed by the value of OD. Data are shown as the means ± SEM (n = 12). Group data (B) and representative images (C,D) are shown. (B) Number of migratory cells per field of LNCaP-control cells and LNCaP-miR-124 cells. Representative photomicrographs illustrating migration of LNCaP-control cells (C) and LNCaP-miR-124 cells (D). Five nude mice per group were injected subcutaneously with 4x10⁶ LNCaP-control cells or LNCaP-miR-124 cells. (E) The volume of xenograft tumors were measured every 10 days from 2 weeks after inoculation. (F) Tumor weights were measured at the time of sacrifice on 34 day after tumor cell injection. (G) Tumors were excised at the time of sacrifice on 34 day post tumor cell injection. Data are presented as means ± SEM. Asterisks indicate P<0.05, double asterisks indicate P<0.001.