<p>HEK293T cell lysates containing the indicated FLAG-tagged TTC proteins were mixed respectively with mouse testis lysates and subjected to co-immunoprecipitation with anti-FLAG M2 resin. Flag-luciferase was used as negative control. Immunoblotting was then performed to detect the FLAG-tagged proteins and representative components of the IFT complexes (A) or BBSome (B).</p
<p>A) Alignment of IFITM3 N-terminal amino acids from various species. Bold and underlined text high...
<p>293 T cells were transduced by Lenti-IRES-GFP or Lenti-Flag-Tax lentiviruses for 72 h. Cells were...
<p>HeLa cells ectopically expressing Flag-tagged BAP, Ank1, or Ank6 and were treated with TNFα or ve...
<p>(A) Immunofluorescence staining. Plasmids encoding the Flag-TTP constructs listed in the figure w...
<p>The immunoprecipitated proteins from testicular TS fraction with either Ts4 or normal control IgM...
(A) SDS-PAGE of crosslinked immunoprecipitants and input from Rbm46FLAG/FLAG and Rbm46WT/WT testes. ...
<p><b>A</b>) A silver stained gel from GFP IP sample of testis lysate. Lane 1 shows the wild-type (W...
<p>(A) Schematic of the domain structure of mouse PABPN1. (B) Pull-down assay of MBP-PABPN1 and WT G...
<p>(A) HEK293T cells were co-transfected with plasmids encoding GFP-TMCC1 and FLAG-tagged TMCC1 frag...
<p>A. Fbxw4 can be immunoprecipitated by ubiquitinated proteins and Fbxw4 can immunoprecipitate ubiq...
<p><b>(A-H)</b> Indirect immunofluorescence of (A-D) apical part of testis and (E-H) spermatocytes s...
<p>(A) A representative gel image showing bands representing proteins immunoprecipitated by the mono...
<p>(<b>A</b>) HEK293 cells were co-transfected with both HA-Foxp3 and Flag-SIRT1, lysed, and Foxp3 w...
<p>(A) HEK-293 cells transfected Flag-tagged DNAJB3 and proteins lysates were coimmunprecipitated wi...
<p><b>A and B.</b> HEK293 cells were transfected with FLAG-GCH1 or empty vector (control) for 2 days...
<p>A) Alignment of IFITM3 N-terminal amino acids from various species. Bold and underlined text high...
<p>293 T cells were transduced by Lenti-IRES-GFP or Lenti-Flag-Tax lentiviruses for 72 h. Cells were...
<p>HeLa cells ectopically expressing Flag-tagged BAP, Ank1, or Ank6 and were treated with TNFα or ve...
<p>(A) Immunofluorescence staining. Plasmids encoding the Flag-TTP constructs listed in the figure w...
<p>The immunoprecipitated proteins from testicular TS fraction with either Ts4 or normal control IgM...
(A) SDS-PAGE of crosslinked immunoprecipitants and input from Rbm46FLAG/FLAG and Rbm46WT/WT testes. ...
<p><b>A</b>) A silver stained gel from GFP IP sample of testis lysate. Lane 1 shows the wild-type (W...
<p>(A) Schematic of the domain structure of mouse PABPN1. (B) Pull-down assay of MBP-PABPN1 and WT G...
<p>(A) HEK293T cells were co-transfected with plasmids encoding GFP-TMCC1 and FLAG-tagged TMCC1 frag...
<p>A. Fbxw4 can be immunoprecipitated by ubiquitinated proteins and Fbxw4 can immunoprecipitate ubiq...
<p><b>(A-H)</b> Indirect immunofluorescence of (A-D) apical part of testis and (E-H) spermatocytes s...
<p>(A) A representative gel image showing bands representing proteins immunoprecipitated by the mono...
<p>(<b>A</b>) HEK293 cells were co-transfected with both HA-Foxp3 and Flag-SIRT1, lysed, and Foxp3 w...
<p>(A) HEK-293 cells transfected Flag-tagged DNAJB3 and proteins lysates were coimmunprecipitated wi...
<p><b>A and B.</b> HEK293 cells were transfected with FLAG-GCH1 or empty vector (control) for 2 days...
<p>A) Alignment of IFITM3 N-terminal amino acids from various species. Bold and underlined text high...
<p>293 T cells were transduced by Lenti-IRES-GFP or Lenti-Flag-Tax lentiviruses for 72 h. Cells were...
<p>HeLa cells ectopically expressing Flag-tagged BAP, Ank1, or Ank6 and were treated with TNFα or ve...