Separation of physical and chemical information in spectra of pollen and spores.

<p>(<b>A</b>) FTIR spectra of <i>Poa pratensis</i>: recorded spectrum (green), scatter estimate with constant, linear, quadratic, and cubic terms (blue), and EMSC corrected spectrum (red) (multiplicative parameter <i>b</i> for <i>Poa pratensis</i> is 1.65); Savitzky–Golay smoothing (quadratic polynomial with window size 11) was obtained prior to EMSC correction with weighting vector. (<b>B</b>) EMSC parameters, and (<b>C</b>) scatter estimates for 10 <i>Poaceae</i> (blue) and 10 <i>Cyperaceae</i> spectra (red). (<b>D</b>) plot for linear and quadratic EMSC parameters for measured samples: Cyperaceae (Cyp), Poaceae (Poa), Cupressaceae (Cup), Pinaceae (Pin), Fagaceae (Fag), Betulaceae (Bet), <i>Geastrum</i> (Gea), <i>Lycoperdon</i> (Lyc), and <i>Scleroderma</i> (Scl).