Expression of P2X6 subunit in adult mice hippocampus.

<p>(<b>a-c</b>) Immunohistochemical analysis of hippocampus sections from 8 month-old mice. Cells were labeled with antibodies against P2X6 (<b>a</b>), P2X4 (<b>b</b>) and P2X2 (<b>c</b>) subunits. Insets depict enlarged views (40X magnification) of CA3 hippocampal region showing a dotted pattern for P2X6 into the nucleus in addition to cytoplasmic staining (<b>a</b>), somatodendritic stainining for P2X4 (<b>b</b>) and axonal and cytoplasmic staining for P2X2(<b>c</b>). Scale bar 200 μm. (<b>d-f</b>) Immuno-electron microscopy analysis of a CA3 hippocampal neuron from 8 months-old wild type mouse labeled with anti-P2X6 antibody. The staining is observed both in the cytoplasm (cyt, white arrows) and into the nucleus (nuc, white asterisks). Scale bar 2 μm. (<b>d-e</b>) Inset of the area indicated in (<b>e</b>) showing a 3.5 X magnification of this indicated area. P2X6 positive regions are mainly located in patches added to the inner nuclear membrane (<b>e</b>).