CDT intoxication promotes cell spreading on fibronectin but not on vitronectin.

<p>HeLa (<b>A</b>) or U2OS (<b>B</b>) cells, left untreated (- CDT) or intoxicated with CDT (1μg/ml) for 6h (+ CDT), were detached with 2.5 mM EDTA in PBS, seeded on fibronectin- or vitronectin-coated glasses for 20 minutes, prior fixation and immunofluorescence analysis. The actin cytoskeleton was visualized by phalloidin staining. Quantification of cell spreading in HeLa (<b>C</b>) and U2OS (<b>D</b>) cells, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0124119#pone.0124119.g001" target="_blank">Fig 1</a>. The graph shows the area of 100 randomly selected adherent cells, measured using the ImageJ software. The data are presented as the mean ± SEM. Statistical analysis was performed using the Student t-test. *** p value < 0.001.