The effect of HCV on human hepatocyte viability via EOR.

<p>(A). Huh7.5.1 cells were infected with JFH1 at a virus titer (IU/cell) of 0, 0.02, 1 and 5. At 48 h postinfection, cell viability was assessed by using Cell Titre-Glo assay. Values are means ± SD (n = 3). * P < 0.05. (B). Huh7.5.1 cells were infected with JFH1 at a virus titer (IU/cell) of 5 and treated with TMB-8 (100 μM) for 4 h, NAC (30 mM) for 8 h and SN50 (40 μM) for 4 h as indicated. Mock-infected Huh7.5.1 cells were used as controls. At 48 h postinfection, cell viability was assessed by using Cell Titre-Glo assay. Values are means ± SD (n = 3). * P < 0.05. (C). Primary human hepatocytes in 96-well plates were infected with JFH1 at a virus titer (IU/cell) of 1 and treated with TMB-8 (100 μM) for 4 h, NAC (30 mM) for 8 h and SN50 (40 μM) for 4 h as indicated. Mock-infected primary human hepatocytes were used as controls. At 48 h postinfectiom, cell viability was assessed using Cell Titre-Glo assay. Values are means ± SD (n = 3). * P < 0.05.