Nicotinamide inhibits neutrophil infiltration of lungs during VILI.

<p>Mice were anesthetized and placed on mechanical ventilation (MV) and received i.p. nicotinamide or PBS as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123460#pone.0123460.g001" target="_blank">Fig 1</a>. All mice were euthanized at the end of 6 hours mechanical ventilation or spontaneous breathing (control mice), which was 5 hours after NAM or PBS administration, and lungs were harvested, fixed, and embedded. Sections of lungs were incubated with GR-1, an antibody for murine neutrophils. Stained neutrophils (white arrows) are white in these reverse images of mice treated with: (A) PBS only; (B) Nicotinamide only; (C) PBS and mechanical ventilation; and (D) Nicotinamide and mechanical ventilation. Images are shown at 10x (left panels) and 40x (right panels) magnifications.