Neonatal human keratinocytes were cultured in CellnTec basal media (containing 0.07mM calcium) or basal media supplemented with 10μM Y-27632.

<p>Cells were assessed for cell viability at each passage, performed at 90% confluence. Trypan blue exclusion assay differentiated between viable and non-viable cells based upon membrane permeability and growth rate calculated as population doubling per day (A). Growth rate is represented graphically as population doubling over days in culture (B). Phase contrast images at 20x magnification demonstrate morphology of primary passage 3 and 10 HEKn cultured in CnT-07 alone and passage 3 and 10 HEKn-CaY supplemented with Y-27632 (C). n = 3 isolations from separate donors. Data is expressed as Mean +/- SEM. Images are representative of three experimental repeats.