Heterologous expression and silencing of <i>PxmALP</i> have obvious effects on susceptibility to Cry1Ac.

<p>(A) Detection of Cry1Ac binding to Sf9 cells expressing PxmALP by immunolocalization. Panels 1 to 3 are differential interference contrast (DIC) microscopy views, while panels 1F to 3F represent the corresponding fluorescence microscopy images. Cells in 3 and 3F were PxmALP-expressing cells treated with PI-PLC before incubation with Cry1Ac. Bound Cry1Ac is detected as green fluorescence in panels 2F and 3F. The scale bar is 20 μm. (B) Susceptibility to Cry1Ac toxin (100 μg/ml) in control, GUS-expressing (GUS) and PxmALP-expressing (PxmALP) Sf9 cells. White bars represent mean mortality in the absence of Cry1Ac, gray bars present mortality observed after 3h incubation with Cry1Ac. (C) Effect of injection with buffer, dsHamALP1, or dsPxmALP on the relative expression of <i>PxmALP</i> compared to non-injected <i>P</i>. <i>xylostella</i> larvae (Control). Both quantitative and qualitative (lower, agarose gel of PCR products) are shown. (D) Susceptibility to two concentrations of Cry1Ac protoxin in <i>P</i>. <i>xylostella</i> larvae injected with buffer, or dsRNA targeting <i>HamALP1</i> (dsHamALP1) or <i>PxmALP</i> (dsPxmALP). Data presented in all the figures are the means and standard errors (SEM) from three biological replicates measured in triplicate. Different letters among the same treatment indicate significant differences (<i>P</i> < 0.05; Holm-Sidak’s test; n = 3).