Subcellular localization of albumin in intercalated cells.

<p>The subcellular distribution of albumin was assessed by immune-gold electron microscopy and double immunofluorescence histochemistry. A) Top panel: overview micrograph of a mouse renal intercalated cell. The red boxes mark the area magnified in the panels below. Lower panels: high magnification micrograph showing albumin immunoreactivity in spherical structures of varying electron density surrounded by membrane in the basal and mid part of the cell. B) Similar labeling of another intercatated cell. Top panel: overview micrograph of a mouse renal intercalated cell. The red box mark the area magnified in the panel below. Lower panel: high magnification micrograph showing albumin immunoreactivity in a spherical structure surrounded by membrane. C) Double immunolabeling for albumin (red) and early endosome marker EEA1 (green). D) Similar labeling for albumin (red) and the late endosome/lysosome marker Cathepsin D (green). DT = distal tubule/collecting duct, PT = proximal tubule.