Effect of IGF1 and ITGB3 signalling on SKOV3 migration as observed by wound healing assay.

<p>(A) No significant increase in migration was observed between controls and PRKCZ-expressing SKOV3 cells in the absence of stimulation (black bars). However, upon treatment with IGF1, parental and vector-control cells showed an increase in migration (white bars; * p<0.05, n = 2). This observation was not seen in PRKCZ-expressing clones PC = parental control; N2 = empty vector control N2. (B, C) Knockdown of <i>ITGB3</i> has no effect on cell migration in SKOV3 cells. Confirmation of <i>ITGB3</i> knockdown in SKOV3 cells (*<i>p<</i>0.01), and distance travelled by SKOV3 cells with and without treatment of <i>ITGB3</i> siRNA (*<i>p<</i>0.05, n = 3).