Effects of PPAR-γ activation on cell proliferation and cell cycle in Ang II-stimulated vascular smooth muscle cells (VSMCs).

<p>Growth-arrested VSMCs were switched to fresh medium containing 0.5% of serum when pretreated with or without PPAR-γ antagonist GW9662 (3 μM), BADGE (1 μM) for 30 min or PPAR-γ specific siRNA for 48 hrs prior to the addition of rosiglitazone (1, 5 and 10 μM) or PPAR-γ nature ligand 15-d-PGJ2 (1, 5 and 10 μM). Ang II (0.1 μM) was then added for 24 hrs. (A). CCK8 assays (values represent the mean ± S.E.M. (n = 12) were carried out to assess cell proliferation. Ros = rosiglitazone; 15d = 15-d-PGJ; Ang II = Angiotensin. *<i>P<0</i>.<i>05</i> vs. control; #<i>P<0</i>.<i>05</i> vs. Ang II; †<i>P<0</i>.<i>05</i> vs. Ang II + Ros.