PIP3 functional analysis and subcellular localization of tumor-associated PTEN mutations.

<p>* PIP3 <i>in vivo</i> activity in yeast was monitored by the reconstitution of the p110α-CAAX-induced lack-of-growth phenotype (+, reconstitution; +/-, partial reconstitution;-, no reconstitution). Data of mutations are on a PTEN 1–403 background, from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0119287#pone.0119287.g002" target="_blank">Fig 2</a> and from [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0119287#pone.0119287.ref036" target="_blank">36</a>].</p><p>† Major subcellular localization (N, nucleus; C, cytoplasm; N/C, nucleus/cytoplasm) was determined by immunofluorescence on mammalian COS-7 cells. Data of mutations are on a PTEN 1–375 background, from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0119287#pone.0119287.g002" target="_blank">Fig 2</a>.</p><p>^# From COSMIC (<a href="http://cancer.sanger.ac.uk/cosmic" target="_blank">http://cancer.sanger.ac.uk/cosmic</a>) and HGMD (<a href="http://www.hgmd.cf.ac.uk" target="_blank">http://www.hgmd.cf.ac.uk</a>) databases. Abbreviations: BD, bladder cancer; BRR, Bannayan-Riley-Ruvalcaba syndrome; CC, colon carcinoma; CS, Cowden syndrome; EC, endometrial cancer; Glb, glioblastoma; JPS, juvenile polyposis syndrome; LC, lung carcinoma; LDD, Lhermitte-Duclos disease; NHML, non-Hodgkin’s malignant lymphoma; OC, ovarian carcinoma; SC, stomach carcinoma; SS, synovial sarcoma. In italic, germ-line mutations.</p><p>PIP3 functional analysis and subcellular localization of tumor-associated PTEN mutations.