Target gene expression following <i>PAX3</i> silencing.

<p>Remaining expression levels (%) of PAX3 downstream target genes in melanocytes (NHEM-n and NHEM-a (I)) and melanoma cells (WM115 and M14) assessed two day post-transfection with <i>PAX3</i> siRNA. Cells were treated with 10 μM siPAX3#1 (s224172, Ambion) alone, with consistent results observed across several independent experiments. Expression levels of downstream target genes were normalised to 18S (ΔCt) and calculated relative to the negative control siRNA (ΔΔCt). Each silencing experiment was performed with a biological duplicate.