Egg-related bacteria and antimicrobials associated with clutch age.

<p>The clutch age is the number of days that the completed clutch spent in the nest; day 1 is the day of clutch completion. Incubation starts between day 1 and day 2 (<i>arrow</i>). ‘r²’ corresponds to the coefficient of determination between clutch age and bacterial/antimicrobial data. (A) Bacterial abundance is examined from day 1 to day 11 (<i>full line</i>) and from day 2 to day 11 (<i>dashed line</i>). (B) Shannon‘s diversity index. (C) Relative abundance of Operational Taxonomic Units (OTUs) of three main classes of <i>Proteobacteria</i>: <i>Alphaproteobacteria</i> (Alpha<i>-</i>) (<i>dotted line</i>), <i>Betaproteobacteria</i> (Beta<i>-</i>) (<i>dashed line</i>) and <i>Gammaproteobacteria</i> (Gamma<i>-</i>) (<i>full line</i>). (D) Albumen pH. (E) Lysozyme (<i>dashed line</i>) and ovotransferrin (<i>full line</i>) concentrations.