Human galectin-2 treatment induces M1 polarization of macrophages in vivo.

<p>(A) Representative immunofluorescence staining of CD40/F4-80 (M1) and CD206/F4-80 (M2) macrophages on muscle sections of the left adductor of the ligated hind limb obtained from placebo and human galectin-2 treated mice at 7 days after occlusion of the femoral artery is shown.Photomicrographs show arteries identified by SMA (yellow; Alexa fluor 488), cell nucleus by Hoechst (blue), macrophages by F4/80 (red; Alexa fluor 647) and macrophage subtypes by CD40 (green; streptavidin Alexa fluor 555) or CD206 (green; streptavidin Alexa fluor 555) staining as described in methods. Arrowheads indicate the macrophage subtypes. (B) Boxplot showing the counts of M1 and M2 macrophages in placebo vs. human galectin-2 treated mice. Data are presented as median and range. Boxes represent the first and third quartiles, the line within the box represents the median, and the lines outside the box represent the spread of the values. N = 7 for placebo, N = 5 for human galectin-2 treated group. (**<i>P</i> < 0.01, Mann-Whitney U test).