Mitochondrial enzyme analyses of the VCP^R155H/+ and WT mouse quadriceps treated with rapamycin or chloroquine.

<p>Quadriceps muscles from <b>(A,D)</b> vehicle control <b>(B,E)</b> rapamycin-treated or <b>(C,F)</b> chloroquine-treated animals were stained with SDH antibody to observe mitochondrial proliferation and oxidative fibers/capacity (Black arrows point to Type II dark fibers; White arrows point to lighter fibers) and <b>(G-L)</b> Oil Red O to observe lipid droplets in WT and VCP^{<b>R155H/+</b>} mice at 20 months of age (Magnification: 400X). Black arrows point to increased Oil Red O Staining; white arrows point to diminished Oil Red O staining. <b>(M)</b> Quantification of Type II oxidative fibers with autophagy-modifying drugs. The number of mice analyzed per experiment is 8–10. Statistical significance is denoted by *<i>p</i><0.005 by Student one-tailed <i>t-</i>test.