<i>pzg</i> mutant cells show characteristics of genuine dying cells and induce AiP.

<p><i>pzg</i> was downregulated in the posterior part of the wing disc. Pzg protein is shown in blue (C, C''), the posterior compartment is marked in green with GFP (A-D; A''-D''; A'''-B'''). (<b>A-A'''</b>) DNA-synthesis visualized with EdU-labeling (red) is amplified anteriorly along the A/P compartment boundary (arrows) upon <i>pzg</i> depletion (A-A') compared to <i>en-</i>Gal4 UAS-<i>GFP</i> control (A'''), whereas a loss can be observed within the posterior compartment (open arrow, A-A'). (<b>B-B'''</b>) Cell division was visualized with anti-PH3 (red). Compared to the control (B'''), many cells in the posterior domain lost this marker upon <i>pzg</i> depletion (arrowhead, B-B'), whereas a strip of cells anterior to the A/P boundary shows stronger PH3 labeling (arrows, B-B'). (<b>C-C''</b>) Expression of Wg protein was monitored (red): it is interrupted at the dorso-ventral boundary in response to <i>pzg</i>-RNAi depletion (arrowhead in C'), whereas ectopic induction of Wg is observed in the posterior compartment outside the normal Wg expression domain (arrows, C'). (<b>D-D''</b>) <i>dpp</i>-lacZ expression (red) is unchanged by <i>pzg</i> depletion in the posterior compartment. Genotypes: (A'''-B''') <i>en-</i>Gal4 UAS-<i>GFP</i>/+. (A-C'') <i>en-</i>Gal4 UAS-<i>GFP</i> UAS<i>-pzg-</i>RNAi/+. (D-D'') <i>en-</i>Gal4 UAS-<i>GFP</i> UAS<i>-pzg-</i>RNAi<i>/</i>+; <i>dpp</i>-lacZ/+. Wing discs are oriented posterior rightwards and dorsally upwards. The A/P compartment boundary is marked with a dotted line. Scale bars represent: (A, B, D) 100 μm, (C) 50 μm.