Depletion of FOXA1 stimulates PR and CHD8 recruitment to PRbs.

<p>(A) Overlapping between progesterone-dependent CHD8 binding sites (green), progesterone-dependent PRbs (red) [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005174#pgen.1005174.ref025" target="_blank">25</a>] and FOXA1 binding sites (blue) in T47D cells (ENCODE dataset GSM803409). (B) ChIP analysis of FOXA1 occupancy at the indicated enhancers in T47-YV or T47D-MTVL cells stimulated with R5020 (R5020) or vehicle (EtOH) for 45 min. (C) Western blot analysis of FOXA1 expression upon transfection of T47D-MTVL cells with control siRNA (siCt) or siRNA against FOXA1 (siFOXA1). (D-E) ChIP analysis of CHD8 (D) and PR (E) at the indicated enhancer in T47D-MTVL cells transfected with control siRNA (siCt) or siRNA against FOXA1 (siFOXA1), and then stimulated with R5020 (R5020) or vehicle (EtOH) for 45 min. (B, D-E) Data are the mean of at least n = 6 qPCR reactions from three independent experiments. Error bars represent ± SD values. * <i>p</i> < 0.01; ** <i>p</i> < 0.001; *** <i>p</i> < 0.0001 using Student’s t-test; n.s., not significant.