CHD8 is required for RNAPII recruitment and eRNA synthesis at PR enhancers.

<p>(A) Time course analysis of PR, CHD8 and RNAPII recruitment to <i>FKBP5e</i>, <i>NFE2L3e</i> and <i>IL6STe</i> enhancers by ChIP. T47D-MTVL cells were stimulated with R5020 (R5020) or vehicle (EtOH) for the indicated times and then processed for ChIP by using antibodies against PR, CHD8 and RNAPII. (B) ChIP analysis of RNAPII enrichment at the indicated enhancers in T47D-MTVL cells transfected with control siRNA (siCt) or siRNA against CHD8 (siCHD8), and then stimulated with R5020 (R5020) or vehicle (EtOH) for 45 min. (C) Expression of enhancer RNAs (eRNA) from the indicated enhancers in T47D-MTVL cells transfected with control siRNA (siCt) or siRNA against CHD8 (siCHD8), and then stimulated with R5020 (R5020) or vehicle (EtOH) for 45 min or 6 h. (A-C) Data are expressed as fold induction relative to the level in ethanol treated cells. Data are the mean of at least n = 6 qPCR reactions from three independent experiments. Error bars represent ± SD values. * <i>p</i> < 0.01; ** <i>p</i> < 0.001; *** <i>p</i> < 0.0001 using Student’s t-test.