Xylan XylT activity measured using fluorescently tagged (AA) xylo-oligosaccharides of different lengths (Xyl<sub>1</sub>-Xyl<sub>6</sub>) as exogenous acceptors.
- Lili Song (589067)
- Wei Zeng (126658)
- Aimin Wu (311661)
- Kelsey Picard (727276)
- Edwin R. Lampugnani (468299)
- Roshan Cheetamun (727277)
- Cherie Beahan (727278)
- Andrew Cassin (468300)
- Andrew Lonsdale (650180)
- Monika S. Doblin (727279)
- Antony Bacic (204169)
Publication date
April 2015
DOIAbstract
<p>The reaction was conducted by mixing <i>Asparagus</i> microsomes with cold UDP-Xyl and the fluorescent acceptors Xyl<sub>1</sub>-AA (B), Xyl<sub>2</sub>-AA (C), Xyl<sub>3</sub>-AA (D), Xyl<sub>4</sub>-AA (F), Xyl<sub>5</sub>-AA (G) or Xyl<sub>6</sub>-AA (H) and incubated at RT for 1 hr. The reaction products were separated by RP-HPLC and detected by a fluorescence detector. A chromatogram showing a separation of a Xyl<sub>1</sub>-AA to Xyl<sub>6</sub>-AA mixture of standards is shown in (A) and (E) for reference. The numbers on the plots indicates the DP of the xylo-AA oligosaccharides.</p
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