<i>tgrC1⁻</i> development is partially restored by suppressor mutations.

<p>A. We developed the suppressor strains (<i>tgrC1⁻stcA^ins</i>, <i>tgrC1⁻stcB^ins</i> and <i>tgrC1⁻stcC^ins</i>), the laboratory wild-type strain AX4 and the parental <i>tgrC1⁻</i> strain on non-nutrient agar. We photographed the cells with light microscopy from above at 16 hour, 24 hour and 72 hour as indicated (strain genotypes are indicated on the left). Scale bar, 1 mm. B. We determined the sporulation efficiency of the same strains at 72 hour of development on black filters. The results are shown as the average (n = 3) and s.d. of the fraction of cells that made spores normalized to the wild type AX4 (%, y-axis). Strain genotypes are indicated on the bottom. C. An illustration of the <i>stcA</i> gene and the insertional mutation. The thick red line represents the two exons and the thin angled lines represent the single intron. The triangle represents the pBSR1 plasmid, which was inserted at the 3’-end of the gene. The inserted plasmid is not drawn to scale. Numbers (bp) below the lines indicate the beginning and the end of the gene model, the predicted splicing borders and the insertion site.