The macroscopic and microscopic observation of cell—scaffold constructs cultured for 48-h <i>in vitro</i>.

<p>(A) Macroscopic images of cell—scaffold constructs cultured for 48-h <i>in vitro</i>. The constructs appeared pink. (B-C) H&E staining of cross sections of constructs. Both AF and NP phases were filled with large amounts of cells (arrows), indicating that cells had a good proliferation in the scaffold. (D-E) SEM of cells in constructs. Both phases were filled with cells (arrows) and large amounts of ECM. (F-G) Live/dead staining of cells in constructs. Cells (arrows) in the constructs displayed green fluorescence, with no red flurescence. (green fluorescence: viable, red fluorescence: necrotic).