MAD-2 is enriched at the nuclear periphery after DNA damage in an ATR- dependent manner.

<p>(A) Wild-type, <i>mad-1(gk2)</i>, <i>chk-1</i>(RNAi), <i>atr(tm853)</i>, and <i>atr(tm853);mad-1</i>(RNAi) germ lines stained with RAD-51 (green) and counterstained with DAPI (magenta). Numbers to right indicate mean RAD-51 foci per germ line ±SEM (n = 10). (B) MAD-2 (green) staining in wild-type or <i>atr(tm853)</i> germ lines in the absence of damage or after HU or IR treatment counterstained with DAPI (magenta). Arrow indicates cell with nuclear MAD-2 staining. (C) Germ lines of <i>mad-3(ok1580)</i> and <i>bub-3</i>(RNAi) worms after HU stained with MAD-2 (red) and NPC (green). (D) MAD-2 localization after IR is ATR and ATM dependent. Although some MAD-2 (red) can still localize to the nuclear periphery (NPC, green) in <i>atm-1(gk186)</i> after 30 gy of IR, MAD-2 localization is abolished in the <i>atm-1(gk186); atr(tm853)</i> double mutant. Scale bars = 10μm.