Both DDR and SAC components are required for efficient DNA damage repair and progeny viability after HU.

<p>(A) Percent of nuclei that contain at least 1 RAD-51 focus—HU or 16 hours after 5mM HU recovery in wild-type, <i>fzy-1(av15)</i>, <i>mad-1(gk2)</i>, <i>mad-2</i>(RNAi), <i>mad-3(ok1580)</i>, <i>bub-3</i>(RNAi), <i>and atr(tm853)</i> worms. Difference between—HU and +HU is statistically different in all genotypes except wild type and <i>fzy-1(av15)</i>, p< 0.02 (two-way ANOVA)(n≥13). (B) Percent progeny inviability—HU and after 5mM HU exposure in WT <i>and mad-3(ok1580)</i>; n≥13. *p<0.05, **p<0.001, ***p<0.0001 (two-way ANOVA). Error bars represent SEM. (C) Proliferative zone nucleus of WT worm stained for RAD-51 (white), NPC (red), MAD-2 (green) and DAPI (blue) in the presence of HU. Image represents one slice of a z-stack taken on SIM. Scale bar = 5μm.