<p><sup>a</sup> Results are expressed in nanomoles of product formed per minute and per milligram of protein in S100 extracts. Induction was performed with vancomycin (0.25 μg/ml). Data are means ± standard deviation obtained from a minimum of three independent extracts.</p><p><sup>b</sup> The <i>P</i><sub><i>spank</i></sub> promoter is constitutive due to low expression in the absence of induction by IPTG.</p><p><sup>C</sup><i>term</i> corresponds to the T4 transcription terminator.</p><p>CAT specific activities of <i>P</i><sub><i>UG</i></sub> promoter in <i>E</i>. <i>coli</i> NR698.</p
This research has investigated the effect that altered supercoiling has on in vivo expression from b...
<div><p>Colicins are toxins that mediate interference competition in microbial ecosystems. They serv...
The engineering of synthetic circuits in cells relies on the use of well-characterized biological pa...
<p><sup>a</sup> Results are expressed in nanomoles of product formed per minute and per milligram of...
Chloramphenicol acetyl transferase (CAT) protein and mRNA levels in E. coli were determined followin...
<p>(<b>A</b>) Units of CAT activity detected in <i>E. coli</i> cell extract, containing different pl...
<p>(<b>A</b>) The midgut samples from synchronized 4<sup>th</sup> instar larvae (30 larvae/sample) w...
<p>(A) Comparison of the P<sub>cat</sub> and the <i>S.</i> Typhimurium (STY) σ<sup>70</sup> consensu...
<p>(A) Dose-response curve of the promoter variants (P<sub>tetA </sub><i>gfp+</i> with P<sub>cat</su...
<p>The CAT activities measured in soluble extracts of cells collected in the mid-exponential growth ...
A novel cross-regulation expression system has been shown previously to be very effective for regul...
<div><p>(A) Gal4-VP16. Left: a CAT reporter construct containing the E1b core promoter and four Gal4...
Promoters are one of the building blocks of a synthetically engineered biological system. Within a s...
<p>A) Measured β-galactosidase activities of strain BW25113, carrying the <i>lac</i> expression vect...
<p>In the promoter/CAT group, <i>hsp70</i> short promoter vector plasmid was added in at 1∶1 ratio t...
This research has investigated the effect that altered supercoiling has on in vivo expression from b...
<div><p>Colicins are toxins that mediate interference competition in microbial ecosystems. They serv...
The engineering of synthetic circuits in cells relies on the use of well-characterized biological pa...
<p><sup>a</sup> Results are expressed in nanomoles of product formed per minute and per milligram of...
Chloramphenicol acetyl transferase (CAT) protein and mRNA levels in E. coli were determined followin...
<p>(<b>A</b>) Units of CAT activity detected in <i>E. coli</i> cell extract, containing different pl...
<p>(<b>A</b>) The midgut samples from synchronized 4<sup>th</sup> instar larvae (30 larvae/sample) w...
<p>(A) Comparison of the P<sub>cat</sub> and the <i>S.</i> Typhimurium (STY) σ<sup>70</sup> consensu...
<p>(A) Dose-response curve of the promoter variants (P<sub>tetA </sub><i>gfp+</i> with P<sub>cat</su...
<p>The CAT activities measured in soluble extracts of cells collected in the mid-exponential growth ...
A novel cross-regulation expression system has been shown previously to be very effective for regul...
<div><p>(A) Gal4-VP16. Left: a CAT reporter construct containing the E1b core promoter and four Gal4...
Promoters are one of the building blocks of a synthetically engineered biological system. Within a s...
<p>A) Measured β-galactosidase activities of strain BW25113, carrying the <i>lac</i> expression vect...
<p>In the promoter/CAT group, <i>hsp70</i> short promoter vector plasmid was added in at 1∶1 ratio t...
This research has investigated the effect that altered supercoiling has on in vivo expression from b...
<div><p>Colicins are toxins that mediate interference competition in microbial ecosystems. They serv...
The engineering of synthetic circuits in cells relies on the use of well-characterized biological pa...