Daam1 interacts with the central region of Piccolo.

<p>(<b><i>A</i></b>) Schematic diagram of Piccolo depicting relative positions of different subdomains and composition of EGFP-tagged cDNA clones used in pull-down assays. <b>(<i>B</i>)</b> Western blot analysis of EGFP-tagged Piccolo fragments and Myc-Daam1 expressed and immunoprecipitated from COS7 cells with an antibody to GFP. (<b><i>C</i></b>) Schematic diagram of Daam1 depicting relative positions of different subdomains and organization of Myc-tagged cDNA clones. <b>(<i>D</i>)</b> Western blot analysis of Myc-tagged Daam1 constructs co-expressed with EGFP-Pclo_1980-2553 and immunoprecipitated from COS7 cells with an antibody to Piccolo. Q—polyQ domain; Zn1 and Zn2—zinc fingers 1 and 2; CC—coiled coil domain; PRS—proline rich sequence; PDZ—PDZ domain; C2—C2 domain; GBD—Rho GTPase binding domain; DID—Diaphanous Inhibitory Domain; FH1 and FH2—formin homology domains 1 and 2; DAD—Diaphanous Autoregulatory Domain.