Comparison of transcriptome changes in BAC-Q72 and Pcp2-ATXN2[Q127] mice.

<p><b>(A)</b> The Venn diagram of transcriptome changes using an FDR ≥15 and Log2 ratio of change ≥|0.3|. Class I transcripts are changed only in BAC-Q72 and class III transcripts changed only in Pcp2-ATXN2[Q127] cerebella. A total of 236 transcripts (class II) are significantly altered in both models. <b>(B)</b> Validation of six overlapping genes (class II) by qRT-PCR. Cerebellar RNAs from BAC-Q72 and WT littermates (both at 8 weeks of age) and Pcp2-ATXN2[Q127] and WT littermates (both at 6 weeks of age) show significant reductions of transcript expression. Genes tested are; <i>Rgs8</i>, <i>Calb1</i>, <i>Pcp2</i>, Purkinje cell protein 4 (<i>Pcp4</i>), Homer homolog 3 (Drosophila) (<i>Homer3</i>) and Centrosomal protein 76 (<i>Cep76</i>). Gene expression levels were normalized to beta-actin. Six animals from each group were used in this experiment. Data are means ± SD, *p<0.05 **p<0.01, ***p<0.001, Student t-test. <b>(C)</b> Fold change relation between RNA-seq data and observed experimental qRT-PCR data are tabulated.