Renalase peptides demonstrate protective effects.

<p>A, amino acid sequence of renalase peptides: RP-Scr220: scrambled RP-220. B, Effect of renalase peptides on survival of HK-2 cells exposed to 20μM cisplatin for 24 hrs;. cell survival is depicted as % change in survival compared to that in cisplatin-treated HK-2 cells without renalase peptides; cell survival measured by the WST-1 method, RP-A220: mutated RP-220, and RP-19 and RP-128: control peptides; peptide concentration (μg/ml) indicated in top line; n = 4, * = p<0.05. C, Comparison of protective effect of recombinant renalase, RP-224, RP-220, and RP-H220 on survival of HK-2 cells exposed to 20μM cisplatin for 24 hrs; cell survival is depicted as % change in survival compared to that in cisplatin-treated HK-2 cells without renalase peptides; n = 4, * = p<0.05. D, Dose response curve for RP-220 and RP-H220; HK-2 cells exposed to 20μM cisplatin for 24 hrs; cell survival is depicted as % change in survival compared to that in cisplatin-treated HK-2 cells without renalase peptides; n = 4, * = p<0.05.