Maximum stabilization and destabilization induced by mutations at each residue position.

<p>(A) Plot comparing the minimum and maximum simulated <i>T</i>_m values, for each residue across all 19 simulated mutants. <i>T</i>_m is normalized to WT, by dividing each <i>T</i>_m value by the simulated WT <i>T</i>_m = 1.489 simulation units. Outliers are circled in purple (left), green (middle) and orange (right). (B) DHFR with outlier residues colored according to the color scheme from (A). Purple: residues F153, W30, Y111, L156, L110. Green: residues A107, I155, L112, H114. Orange: residues A6, E154. Excluding outlier residues, the C-terminal beta hairpin is colored yellow, and the rest of the protein is colored cyan.