<p>Dependence of the initial reaction rate on the substrate concentration (molinate, top; thiobencarb, bottom) by recombinant molinate hydrolase (♦), mutant Arg187Ala (●) and mutant Phe253Ala (■).</p
Previous mechanistic and crystallographic studies on two C - C hydrolase enzymes, Escherichia coli M...
Two serine hydrolases, Candida antarctica Lipase B (CALB) and para-nitrobenzyl (Bs2) esterase from B...
<p>Kinetic parameters of OPH mutants in the DFP hydrolysis with or without triethanolamine (TEA).</p
<p>Detail view of the active site highlighting (in yellow) the residues that potentially restrict th...
<p>Proposed catalytic mechanism for molinate hydrolase based on previous models [<a href="http://www...
Molinate is a recalcitrant thiocarbamate used to control grass weeds in rice fields. The recently de...
<p>(A) Wild-type bglTm. (B) Mutant T1. (C) Mutant T2. The tested substrates were <i>p</i>-nitropheny...
<p>Apparent kinetic parameters for hydrolysis of chloropropionate substrates by wild type and mutant...
In nature, recalcitrant polysaccharides such as chitin and cellulose are degraded by glycoside hydro...
<p>Assays were performed with 0.5 mM molinate in 50 mM phosphate buffer at pH 7.4 at 30°C, with a pr...
<p>The rate of substrate Suc-LLVY-AMC hydrolysis (a release of AMC molecule) with control and inhibi...
A new pathway of molinate mineralization has recently been described. Among the five members of the ...
<p>Kinetic parameters for the substrate hydrolysis by the wild-type and mutant Sfβgly.</p
2To whom correspondence should be addressed Molecular recognition and site-directed mutagenesis are ...
<p>n.d. = below limit of detection; error bars are the standard deviation of 3 replicates.</p><p>H...
Previous mechanistic and crystallographic studies on two C - C hydrolase enzymes, Escherichia coli M...
Two serine hydrolases, Candida antarctica Lipase B (CALB) and para-nitrobenzyl (Bs2) esterase from B...
<p>Kinetic parameters of OPH mutants in the DFP hydrolysis with or without triethanolamine (TEA).</p
<p>Detail view of the active site highlighting (in yellow) the residues that potentially restrict th...
<p>Proposed catalytic mechanism for molinate hydrolase based on previous models [<a href="http://www...
Molinate is a recalcitrant thiocarbamate used to control grass weeds in rice fields. The recently de...
<p>(A) Wild-type bglTm. (B) Mutant T1. (C) Mutant T2. The tested substrates were <i>p</i>-nitropheny...
<p>Apparent kinetic parameters for hydrolysis of chloropropionate substrates by wild type and mutant...
In nature, recalcitrant polysaccharides such as chitin and cellulose are degraded by glycoside hydro...
<p>Assays were performed with 0.5 mM molinate in 50 mM phosphate buffer at pH 7.4 at 30°C, with a pr...
<p>The rate of substrate Suc-LLVY-AMC hydrolysis (a release of AMC molecule) with control and inhibi...
A new pathway of molinate mineralization has recently been described. Among the five members of the ...
<p>Kinetic parameters for the substrate hydrolysis by the wild-type and mutant Sfβgly.</p
2To whom correspondence should be addressed Molecular recognition and site-directed mutagenesis are ...
<p>n.d. = below limit of detection; error bars are the standard deviation of 3 replicates.</p><p>H...
Previous mechanistic and crystallographic studies on two C - C hydrolase enzymes, Escherichia coli M...
Two serine hydrolases, Candida antarctica Lipase B (CALB) and para-nitrobenzyl (Bs2) esterase from B...
<p>Kinetic parameters of OPH mutants in the DFP hydrolysis with or without triethanolamine (TEA).</p
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