NSDV N protein distribution in caecum, duodenum and lung of infected sheep.

<p>Tissue samples were taken post-mortem from animals infected with the NSDVi isolate in a study previously described [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0124966#pone.0124966.ref018" target="_blank">18</a>], or from healthy animals that were not subject to any experimental procedures. Cryosections were prepared and sections were fixed and stained as described in Methods, using mouse monoclonal anti-collagen IV antibody (Coll) and affinity-purified rabbit anti-NSDV N protein antibodies (NSDV N), followed by AlexaFluor 488 goat anti-mouse IgG (green) and AlexaFluor 568 goat anti-rabbit IgG (red). DAPI was used as a counterstain (blue). Scale bars indicate 40 μm (A, B, D, E, G, H) or 10 μm (C, F, I).