Lipid spectrum accumulated in batch culture is dynamic.

<p>Intracellular FAMEs and intact lipids were extracted at 12 hour intervals. (A) Extractable FAMEs accumulate to a maximum level at 48 h and drop in concentration as extracellular glucose is depleted. (B) Intact lipid z-scores were clustered using a Euclidian approach. PE, phosphatidylethanolamine; PC, phosphatidylcholine; TG, triacylglycerol; DG, diacylglycerol; CR, ceramide; FA, free fatty acid; UN, unknown headgroup. Underlines indicate intact lipids identified in negative ESI mode while lack of an underline indicates those identified in positive ESI mode. *Intact lipids that changed significantly during the time course by ANOVA (p < 0.01).