Cellular responses to PO in the myocardium.

<p>WT mice were Sham operated or subject to aortic banding (MTAB) and sacrificed after the indicated time points. Transmural blocks of the left ventricular myocardium were sectioned and immunostained for (A) α-SMA (SMA, blue), desmin (des, red), vimentin (vim, green) and nuclei (DAOI, white) or (B) α-SMA (SMA, green), desmin (des, red), and nuclei (DAOI, blue). Note that exposure time for α-SMA imaging was adjusted in (A) to the high staining intensity in vascular smooth muscle and in (B) to the comparably lower staining intensity in cardiomyocytes overloaded myocardium (~2-times longer exposure). Scale bars: 50 μm.